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…also be done experimentally. The cells must be incubated on ice for 12-24 hours in a calcium chloride solution. The solution punches holes in the cells so the plasmid can enter. Competency is extremely necessary for a successful transformation…

…into the ship Main Hatch- The hatch which led to the main passage Hatchway- A small connecting hole in a wall between two rooms, or the small doors that cover this hole P. 59 Below- Any place below the main deck Longboat- The longest boat, usually a seaworthy rowing…

…Soldiers in the West. They also guarded water holes, railroad construction workers, and horses and cattle. The Buffalo Soldiers led a very dangerous life on the open and unmanaged plains of the west. Another dangerous job they performed was mapping out…

…hardwood hammocks, slash pine forests, and the saltwater mangrove ecotore. As a result it has become part of the Everglade’s largest wetlands restoration efforts. The Hole-in-the-Doughnut, a 4,000-hectare of abandoned farmland, is where the restoration is taking…

…that Rosebud represented. Unable to love, Kane lived life with a gaping hole in his heart. A hole like the missing piece of a puzzle.…

…, and hydrogen oxide radicals are also primarily dangerous. As a result, the Ozone in the stratosphere has been reduced to such an extent that ozone holes are appearing around the globe, in particular one over Antarctica that in 1995 measured 8.2 million square miles…

…hadn’t really valued when he could actually do it. He also has to deal with not being able to walk either. But, if he only knew what he was to later discover. “The hole began at the base of his throat just below where his jaw should be and went upward…

…radia! tion breaks them down. When that happens, the CFC molecule releases atomic chlorine. One chlorine atom can destroy over 100,000 ozone molecules. When this happens, holes are created. The hole over Antarctica is now about 9 million square miles…

…coming from within. I climbed around to the top of the tree and peeked through a hole in the trunk, inside there were seven little partimagin chicks playing around. Sensing a good time, I gnawed the hole bigger and pulled my self in, I started to roll…

…was in town, Huck found a way to escape. He made a hole going through the floor and crawled out. He stages his own murder for a successful and dramatic getaway. A canoe, which Huck discovered a few days earlier, helps him to escape to the deep forest. The next day…