spectrophotometery

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The purpose of this lab experiment is to understand the uses of spectrophotometry. This experiment concentrates on the uses of the spectrophotometer, and using this instrument to specifically determine the absorption spectrum of a cobalt chloride solution, a galactose solution, and to determine an unknown concentration of a galactose solution. Modern biologists frequently use the measurement of light absorption to determine concentration of chemicals. The technique is called spectrophotometry. However, why is light absorbed? Light …

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…is 0.335. Spectrophotometry is important in science today because it shows the peak at which a chemical absorbs the most light. The absorption spectrum can be used almost like fingerprints, identifying unknown chemicals. It is also important because it can determine unknown concentrations in solutions, say for example, the amount of cocaine in a persons blood. For these reasons, it is possible to see why spectrophotometry is one of the most widely used techniques in biology.