To investigate the effect of surface area on the rate of reaction using the enzyme Catalase, found in potato, and the substrate Hydrogen Peroxide.

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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions, without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a 'ball' shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme, …

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…initial rate of reaction. For both methods you have to first display the results in a graph with time on the x-axis and total volume of oxygen collected on the y-axis. Once this is done, you can either, calculate the slope of a tangent to the curve as close to time 0 as possible. Or the simplest way is to read off the graph the amount of oxygen released in the first 30 seconds of the reaction.