The Identification of an Unknown Plasmid using Restriction Enzymes, Gel Electrophoresis, and Transformation. Abstract Using DNA technology, including restriction enzymes, gel electrophoresis, and transformation we performed two experiments to identify an unknown plasmid. The first experiment involved gel electrophoresis of plasmid DNA with and with out restriction enzymes to determine migration and number of base pairs in specific fragments. Known DNA fragments were used to determine the size of the unknown fragments. Plasmid maps of …
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showed last 75 words of 3473 total
…technique. Bibliography 1.) Australian Biotechnology Association. What is Genetic Engineering? Australian Biotechnology Association Home Page. Hotbot. http://www.aba.asn.au/leaf2.html 2.) Campbell, Neil. 1996. Biology, 4th edition. p.338-339, 369 Benjamin/Cummings Publishing Co., Inc. New York. 3.) Johnson, Shea. Recombinant DNA. Student Reports of BI 220 University of Oregon. Infoseek. http://ponderosa-pine.uoregon.edu/Bi220/Johnson/menu.html 4.) Lawrence, S.M., M.K. Heidemann, and D.O. Straney. 2000. Biological Sciences 111L Laboratory Manual, 4th edition. p.141-148