Fluorescence study of dyes
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Words: 838
Pages: 3
(approximately 235 words/page)
Pages: 3
(approximately 235 words/page)
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Fluorescence Study of the
Kinetics of Energy
Transfer Between Dyes
1. Introduction
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra.
For fluorescence spectra, there are two kinds, the excitation and the emission spectrum.
The excitation spectrum is obtained
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showed first 75 words of 838 total
showed last 75 words of 838 total
gives us the following graph. Values obtained: JDA = 6.897x1031 nm mol -1 R0 = 0.14 nm (R0)eff = 0.584 nm based on equation (A3-13) So we see that those are very clearly quite different, and that could show that it does not obey to Forster's theory. 3. Conclusion So we have shown that the quenching of Coumarin 1 by Sodium fluorescein is obeyed by Stern-Volmer kinetics, that there is mainly dipole-dipole transfer, but could not agree with Forster's theory.
gives us the following graph. Values obtained: JDA = 6.897x1031 nm mol -1 R0 = 0.14 nm (R0)eff = 0.584 nm based on equation (A3-13) So we see that those are very clearly quite different, and that could show that it does not obey to Forster's theory. 3. Conclusion So we have shown that the quenching of Coumarin 1 by Sodium fluorescein is obeyed by Stern-Volmer kinetics, that there is mainly dipole-dipole transfer, but could not agree with Forster's theory.