BACTERIAL CONJUGATION EXPERIMENT INTRODUCTION: Bacteria, in general, reproduce asexually, but in order to increase diversity, they have developed a mechanism for transfer of genetic material from one bacterium to another. The ability to perform this transfer is conferred by a set of genes which are called F for 'fertility.' These genes exist on a small, circular piece of deoxyribonucleic acid (DNA) that replicates independently from the bacterial chromosome, or they can be integrated into …
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…minutes 14 NG TNTC NG Histadine deficient plates Time 10-1 10-2 10-3 10-4 0 minutes 10 2 NG NG 10 minutes 46 43 26 3 20 minutes TNTC 136 NG 4 30 minutes TNTC 184 8 3 40 minutes TNTC 340 20 NG 50 minutes 45 TNTC 27 3 60 minutes 41 250 42 NG TNTC = Too numerous to count NG = No growth Figure 4 - Time of entry table (percentages of recombinants) Time Thr - Pro - His - 0 minutes .44 0 0 10 minutes 5.8 2 .02 20 minutes 6.7 2.6 .06 30 minutes .004 1.8 .08 40 minutes .0098 4.3 .09 50 minutes 1.9 2.3 .002 60 minutes 2.4 0 .11 Figure 5 - Time of entry graph (percentages of recombinants) Figure 6 - Map of E. coli K12